Diclofenac sodium is chemically known as 2-[(2, 6-dichlorophenyl)amino] phenyl acetic acid monosodium salt which is a potent non-steroidal anti-inflammatory agent. A novel, accurate and precise method developed and validated for estimation of Diclofenac sodium in its pure form & in their pharmaceutical formulation. The proposed developed method is based on reaction between Diclofenac Sodium and 2, 3-Dichloro-1, 4-napthaquinone (Dichlone) in methanol medium. This reaction was carried out by heating at 600C for 10 minutes which produce a charge transfer complex having a absorbance maxima at 535 nm. The values of optimal reaction conditions such as reagent concentration, heating time and stability of the reaction product were determined. In this work, Beer’s law was followed in a concentration range from of 7.4×10-4 to 1.9×10-3 mol l-1 with a correlation coefficient of 0.999 and molar absorptivity of 0.222×103 l mol-1 cm-1. The limit of detection (LOD) and the limit of quantification (LOQ) were found to be 4.77×10-5 mol l-1 & 1.4×10-4 mol l-1 respectively. In the presence of the common exicipients such as glucose, lactose, talc, starch, magnesium stearate, sodium sulphite, titanium dioxide, polyethylene glycol, polyvinyl pyrrolidone, mannitol and benzyl alcohol, no interferences were observed. Recoveries of Diclofenac sodium from various pharmaceutical preparations were found within range of 98.9 to 101.7%.
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